Composite

Part:BBa_K1647018:Design

Designed by: Yun Xue   Group: iGEM15_WHU-China   (2015-09-15)

CAP-Bac8c, an improved antimicrobial peptide selectively


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To reduce the effect of the CAP on the Bac8c, the CAP only has the least effective sequence of the CSP. Besides, we design an intersequence, GGG, put between the CAP and Bac8c for the same goal.

Source

From the paper, Deciphering the Mode of Action of the Synthetic Antimicrobial Peptide Bac8c. E. C. Spindler, J. D. F. Hale, T. H. Giddings, Jr., R. E. W. Hancock, and R. T. Gill. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2011, p. 1706-1716.

and the paper, Target Killing of Streptococcus mutans by a Pheromone-Guided “Smart” Antimicrobial Peptide. Randal Echert, Jian He, Daniel K. Yarbrough, Fengxia Qi, Maxwell H. Anderson, and Wenyuan Shi. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 2006, p. 3651-3657.

References

Deciphering the Mode of Action of the Synthetic Antimicrobial Peptide Bac8c. E. C. Spindler, J. D. F. Hale, T. H. Giddings, Jr., R. E. W. Hancock, and R. T. Gill. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2011, p. 1706-1716.

Target Killing of Streptococcus mutans by a Pheromone-Guided “Smart” Antimicrobial Peptide. Randal Echert, Jian He, Daniel K. Yarbrough, Fengxia Qi, Maxwell H. Anderson, and Wenyuan Shi. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 2006, p. 3651-3657.